VARIABILITY OF CAROTENOID-SYNTHETIC STRAINS STREPTOMYCES GLOBISPORUS 1912 AFTER DEEP CULTIVATION AND STORAGE
نویسندگان
چکیده
منابع مشابه
Plasmid variability in the istamycin producing strains of Streptomyces tenjimariensis.
Three strains of istamycin-producing Streptomyces tenjimariensis were isolated over a period of time from soils at the same location and were found to have three different types of plasmid profiles. Protoplast fusion between two of these strains provided a clone harboring a smaller plasmid not present in the parent strains. None of the plasmids had restriction sites for EcoR I and Hind III. Mos...
متن کاملDNA-binding activity of LndI protein and temporal expression of the gene that upregulates landomycin E production in Streptomyces globisporus 1912.
The gene lndI is involved in the pathway-specific positive regulation of biosynthesis of the antitumour polyketide landomycin E in Streptomyces globisporus 1912. LndI was overexpressed in Escherichia coli as a protein C-terminally fused to the intein-chitin-binding-domain tag and purified in a one-step column procedure. Results of in vivo LndI titration, DNA gel mobility-shift assays and promot...
متن کاملExpression of the regulatory protein LndI for landomycin E production in Streptomyces globisporus 1912 is controlled by the availability of tRNA for the rare UUA codon.
The gene lndI encodes the activator of landomycin biosynthesis. The utilization of LndI-EGFP fusions led us to investigate the temporal pattern of this gene expression and demonstrated the delay between lndI transcription and translation. The TTA codon in lndI is thought to be the reason for this delay. The replacement of TTA with CTC cancelled the pause between lndI transcription and the trans...
متن کاملassessment of deep word knowledge in elementary and advanced iranian efl learners: a comparison of selective and productive wat tasks
testing plays a vital role in any language teaching program. it allows teachers and stakeholders, including program administrators, parents, admissions officers and prospective employers to be assured that the learners are progressing according to an accepted standard (douglas, 2010). the problems currently facing language testers have both practical and theoretical implications but the first i...
Generation of Streptomyces globisporus SMY622 strain with increased landomycin E production and it's initial characterization.
Landomycin E (LaE) overproducing strain Streptomyces globisporus SMY6222 has been developed using UV induced mutagenesis and selection for streptomycin resistance. SMY622 has been shown by HPLC to produce 200-fold higher amounts of LaE when comparing with parental strain. The levels of transcription of regulatory gene lndI and oxygenase gene lndE are two times higher in the mutant than in the w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbiology&Biotechnology
سال: 2018
ISSN: 2307-4663,2076-0558
DOI: 10.18524/2307-4663.2018.3(43).142234